Determination of polysorbates in foods by solid-phase extraction and high-performance liquid chromatography].

A simple and rapid method using refractive index high-performance liquid chromatography (RI-HPLC) was developed for the determination of polysorbates (PS) in processed foods. PS were extracted with ethyl acetate containing 5% methanol. The extract was cleaned up on a multimode cartridge (300 mg) and an Alumina-N cartridge (500 mg) to remove fats and food color. HPLC separation was performed on a C18 column (4.6 i.d. x 150 mm) with methanol as the mobile phase. The recoveries of PS80 from nine kinds of foods fortified at the levels 1-5 g/kg were 80-99%. The limit of quantitation for PS80 in foods was 0.10 g/kg. The proposed method was applied to Worcestershire sauce that was PS-positive by TLC, and PS was confirmed to be present as PS80 at the concentration of 0.13 g/kg.

Citation

Chie Nomura, Mikiya Kitagawa, Masaharu Yoshida, Yukio Tanaka. Determination of polysorbates in foods by solid-phase extraction and high-performance liquid chromatography]. Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan. 2007 Jun;48(3):64-8

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PMID: 17657999

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