Mutasynthesis-derived myxalamids and origin of the isobutyryl-CoA starter unit of myxalamid B.

Helge B Bode, Peter Meiser, Thorsten Klefisch, Niña Socorro d j Cortina, Daniel Krug, Anke Göhring, Gertrud Schwär, Taifo Mahmud, Yasser A Elnakady, Rolf Müller

Institut für Pharmazeutische Biotechnologie, Universität des Saarlandes, Postfach 151150, 66041 Saarbrücken, Germany. h.bode@mx.uni-saarland.de

Chembiochem : a European journal of chemical biology 2007 Nov 23

filter terms:

Myxalamids are potent inhibitors of the eukaryotic electron transport chain produced by different myxobacteria. Here, we describe the identification of the myxalamid biosynthesis gene cluster from Myxococcus xanthus. Additionally, new myxalamids (5-13) have been obtained by mutasynthesis from bkd mutants of M. xanthus and Stigmatella aurantiaca. Moreover, as these bkd mutants are still able to produce myxalamid B (2), the origin of the isobutyryl-CoA (IB-CoA) starter unit required for its biosynthesis has been determined. In a M. xanthus bkd mutant, IB-CoA originates from valine, but in S. aurantiaca this starter unit is derived from alpha-oxidation of iso-odd fatty acids, thereby connecting primary and secondary metabolism.

Citation

Helge B Bode, Peter Meiser, Thorsten Klefisch, Niña Socorro d j Cortina, Daniel Krug, Anke Göhring, Gertrud Schwär, Taifo Mahmud, Yasser A Elnakady, Rolf Müller. Mutasynthesis-derived myxalamids and origin of the isobutyryl-CoA starter unit of myxalamid B. Chembiochem : a European journal of chemical biology. 2007 Nov 23;8(17):2139-44