Telomere DNA conformation change induced aggregation of gold nanoparticles as detected by plasmon resonance light scattering technique.

Cheng Zhi Huang, Qie Gen Liao, Li Hua Gan, Feng Ling Guo, Yuan Fang Li

College of Pharmaceutical Sciences, CQKL-LRTA, Southwest University, Chongqing 400715, China. chengzhi@swu.edu.cn

Analytica chimica acta 2007 Dec 5

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It has been reported that adsorption of uncoiled DNA (u-DNA) on the surface of gold nanoparticles (Au-NPs) can prevent the nanoparticle suspensions from aggregation even if in salt medium. Herein we report that quadruplex DNA (q-DNA), which is formed from uncoiled telomere DNA, via intramolecular hydrogen bonds in the presence of potassium ion, cannot keep Au-NPs stable, and the q-DNA/Au-NPs coexisting suspensions display aggregation tendency, giving plasmon resonance light scattering (PRLS) signals of Au-NPs. Mechanism investigations through a single point energy calculation on u- and q-structures of telomere DNA showed that q-DNA, compared with u-DNA, has a much higher surface negative charge density, symmetrical charge distribution and well self-structural stabilization, could not be adsorbed on the surface of Au-NPs.

Citation

Cheng Zhi Huang, Qie Gen Liao, Li Hua Gan, Feng Ling Guo, Yuan Fang Li. Telomere DNA conformation change induced aggregation of gold nanoparticles as detected by plasmon resonance light scattering technique. Analytica chimica acta. 2007 Dec 5;604(2):165-9