K Katayama, Jamhari, T Mori, S Kawahara, K Miake, Y Kodama, M Sugiyama, Y Kawamura, T Nakayama, M Maruyama, M Muguruma
Dept. of Biochemistry and Applied Biosciences, Faculty of Agriculture, Univ. of Miyazaki, Miyazaki 889-2192, Japan.
Journal of food science 2007 NovCrude myosin light chain was extracted from Japanese domestic pork loin and digested with pepsin. Antihypertensive peptide was isolated from this digest as a measure of its inhibitory activity for angiotensin-I converting enzyme (ACE). Through isolation with some chromatographies, a single active fraction was isolated, and it was detected as an octapeptide, Val-Lys-Lys-Val-Leu-Gly-Asn-Pro, from 47th to 54th positions of myosin light chain. The 50% inhibitory concentration of this peptide was 28.5 muM. Kinetic evaluation showed that this peptide was a noncompetitive inhibitor, but it was slowly hydrolyzed by ACE. At the dose of 10 mg/kg, this peptide showed antihypertensive activity after a maximum of 3 h of administration and was estimated as a temporally effective hypotensor.
K Katayama, Jamhari, T Mori, S Kawahara, K Miake, Y Kodama, M Sugiyama, Y Kawamura, T Nakayama, M Maruyama, M Muguruma. Angiotensin-I converting enzyme inhibitory peptide derived from porcine skeletal muscle myosin and its antihypertensive activity in spontaneously hypertensive rats. Journal of food science. 2007 Nov;72(9):S702-6
PMID: 18034756
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