Joanna Kowalska, Magdalena Lewdorowicz, Joanna Zuberek, Elzbieta Bojarska, Janusz Stepinski, Ryszard Stolarski, Edward Darzynkiewicz, Jacek Jemielity
Department of Biophysics, Institute of Experimental Physics, Warsaw University, Zwirki i Wigury 93, Warsaw 02-089, Poland.
Nucleosides, nucleotides & nucleic acids 2007Enzymatic cleavage of the P-chiral diastereoisomers of the 5' mRNA cap analogue bearing phosphorothioate moiety in alfa position of 5',5'-triphosphate bridge (m(7)Gppp(S)G D1 and D2) was performed by human Decapping Scavenger (DcpS) enzyme. Analysis of the degradation products allowed to estimate the absolute configuration at the asymmetric phosphorus atoms in examined compounds via correlation with the R(P) and S(P) diastereoisomers of guanosine 5'-O-(1-thiodiphosphate) (GDPalphaS).
Joanna Kowalska, Magdalena Lewdorowicz, Joanna Zuberek, Elzbieta Bojarska, Janusz Stepinski, Ryszard Stolarski, Edward Darzynkiewicz, Jacek Jemielity. Assignment of the absolute configuration of P-chiral 5' mRNA cap analogues containing phosphorothioate moiety. Nucleosides, nucleotides & nucleic acids. 2007;26(10-12):1301-5
PMID: 18066772
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