Expression of a novel 90-kDa protein, Lsd90, involved in the metabolism of very long-chain fatty acid-containing phospholipids in a mitosis-defective fission yeast mutant.

Kazuaki Yokoyama, Miyuki Nakagawa, Masayuki Satoh, Shigeaki Saitoh, Naoshi Dohmae, Ayako Harada, Noriko Satoh, Ken Karasawa, Koji Takio, Mitsuhiro Yanagida, Keizo Inoue

Faculty of Pharmaceutical Sciences, Teikyo University, Suarashi, Sagamiko-machi, Sagamihara-shi, Kanagawa, Japan. yokoyama@pharm.teikyo-u.ac.jp

Journal of biochemistry 2008 Mar

filter terms:

The fission yeast lsd1/fas2 strain carries a temperature-sensitive mutation of the fatty-acid-synthase alpha-subunit, exhibiting an aberrant mitosis lsd phenotype, with accumulation of very-long-chain fatty-acid-containing phospholipid (VLCFA-PL). A novel 90-kDa protein, Lsd90 (SPBC16E9.16c), was found to be newly expressed in small particle-like structures in lsd1/fas2 cells under restrictive conditions. Two mismatches leading to a double frame shift were found between the sequences of the lsd90(+) gene registered in the genomic database and the sequences determined experimentally at the amino acid, cDNA and genomic DNA levels. Unexpectedly, overexpression and disruption of the lsd90(+) gene in either lsd1/fas2 or wild-type cells did not affect either cell growth or expression of the lsd phenotype. The amounts of VLCFA-PL that accumulated in lsd90-overexpressing lsd1/fas2 cells were significantly lower than those in lsd1/fas2 cells, suggesting the involvement of Lsd90 in the metabolism of VLCFA-PL.

Citation

Kazuaki Yokoyama, Miyuki Nakagawa, Masayuki Satoh, Shigeaki Saitoh, Naoshi Dohmae, Ayako Harada, Noriko Satoh, Ken Karasawa, Koji Takio, Mitsuhiro Yanagida, Keizo Inoue. Expression of a novel 90-kDa protein, Lsd90, involved in the metabolism of very long-chain fatty acid-containing phospholipids in a mitosis-defective fission yeast mutant. Journal of biochemistry. 2008 Mar;143(3):369-75