Effect of carbon sources on the induction of xylanolytic-cellulolytic multienzyme complexes in Paenibacillus curdlanolyticus strain B-6.

Rattiya Waeonukul, Khin Lay Kyu, Kazuo Sakka, Khanok Ratanakhanokchai

School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkuntien, Bangkok 10150, Thailand.

Bioscience, biotechnology, and biochemistry 2008 Feb

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The effect of polymeric substances such as alpha-cellulose, birchwood xylan, corn hull, and sugarcane bagasse, and of soluble sugars such as L-arabinose, D-galactose, D-glucose, D-xylose, and cellobiose, on the induction of multienzyme complexes in a facultatively anaerobic bacterium, Paenibacillus curdlanolyticus B-6, was investigated under aerobic conditions. Cells and culture supernatants of strain B-6 grown on different carbon sources were analyzed. Cells grown on each carbon source adhered to cellulose. Hence strain B-6 cells from all carbon sources must have an essential component responsible for anchoring the cells to the substrate surfaces. Native-polyacrylamide gel electrophoresis (native-PAGE), sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), zymogram analysis, and enzymatic assays indicated that many proteins having xylanolytic and cellulolytic activities from P. curdlanolyticus B-6 grown on each carbon source were produced as two multienzyme complexes in the culture supernatants. These results indicate that P. curdlanolyticus B-6 produced multienzyme complexes when grown on both polymeric and soluble sugars. The multienzyme complexes of P. curdlanolyticus B-6 consisted of the main enzymes and non-enzymatic subunits and the production of some different subunits, depending on the carbon source.

Citation

Rattiya Waeonukul, Khin Lay Kyu, Kazuo Sakka, Khanok Ratanakhanokchai. Effect of carbon sources on the induction of xylanolytic-cellulolytic multienzyme complexes in Paenibacillus curdlanolyticus strain B-6. Bioscience, biotechnology, and biochemistry. 2008 Feb;72(2):321-8