IMPY, a potential beta-amyloid imaging probe for detection of prion deposits in scrapie-infected mice.

Pu-Jiao Song, Serge Bernard, Pierre Sarradin, Jackie Vergote, Céline Barc, Sylvie Chalon, Mei-Ping Kung, Hank F Kung, Denis Guilloteau

INSERM, U619, F-37000 Tours, France.

Nuclear medicine and biology 2008 Feb

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A potential single-photon emission computed tomography imaging agent for labeling of A beta plaques of Alzheimer's disease, IMPY (2-(4'-dimethylaminophenyl)-6-iodo-imidazo[1,2-a]pyridine), would be effective in detection of prion amyloid deposits in transmissible spongiform encephalopathies (TSEs). In vitro autoradiographic studies were carried out with [125 I]IMPY on brain sections from scrapie-infected mice and age-matched controls. Competition study was performed to evaluate the prion deposit binding specificity with nonradioactive IMPY. Binding of [125 I]IMPY was observed in infected brain sections, while on age-matched control brain sections, there was no or very low labeling. Prion deposit binding was confirmed by histoblots with prion protein-specific monoclonal antibody 2D6. In the presence of nonradioactive IMPY, the binding of [125 I]IMPY was significantly inhibited in all regions studied. These findings indicate that IMPY can detect the prion deposits in vitro in scrapie-infected mice. Labeled with 123 I, this ligand may be useful to quantitate prion deposit burdens in TSEs by in vivo imaging.

Citation

Pu-Jiao Song, Serge Bernard, Pierre Sarradin, Jackie Vergote, Céline Barc, Sylvie Chalon, Mei-Ping Kung, Hank F Kung, Denis Guilloteau. IMPY, a potential beta-amyloid imaging probe for detection of prion deposits in scrapie-infected mice. Nuclear medicine and biology. 2008 Feb;35(2):197-201