Expression change of beta-1,4 galactosyltransferase I, V mRNAs and Galbeta1,4GlcNAc group in rat sciatic nerve after crush.

Glycosylation is one of the most important post-translational modifications. It is clear that the single step of beta-1,4-galactosylation is performed by a family of beta-1,4-galactosyltransferases (beta-1,4-GalTs), and that each member of this family may play a distinct role in different tissues and cells. beta-1,4-GalT I and V are involved in the biosynthesis of N-linked oligosaccharides. In the present study, Real-time PCR revealed that the beta-1,4-GalT I and V mRNAs reached peaks at 2 w after sciatic nerve crush. In situ hybridization showed that at 1 d after sciatic nerve crush, the expression levels of beta-1,4-GalT I and V mRNAs were strong at the crush site, and decreased gradually from crush site to the distal segments. In addition, combined in situ hybridization for beta1,4-GalT I and V mRNAs and immunohistochemistry for S100 showed that beta1,4-GalT I and V mRNAs were mainly located in Schwann cells. Lectin blot showed that the expression of Galbeta1,4GlcNAc group increased at 6 h immediately, reached a peak at 12 h and remained elevated up to 4 w after sciatic nerve crush. In conclusion, beta1,4-GalT I and V might play important roles in the regeneration of the injured sciatic nerve, and upregulation of Galbeta1,4GlcNAc group might be correlated with the process of the sciatic nerve injury.

Citation

Meijuan Yan, Chun Cheng, Xiaoyi Shao, Ji Qian, Aiguo Shen, Chunlin Xia, Chun Xia. Expression change of beta-1,4 galactosyltransferase I, V mRNAs and Galbeta1,4GlcNAc group in rat sciatic nerve after crush. Journal of molecular histology. 2008 Jun;39(3):317-28

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PMID: 18320333

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