Enzymatic preparation of cefaclor with immobilized penicillin acylase.

Enzymatic syntheses of cefaclor by immobilized penicillin acylase under kinetic control were carried out. According to the initial reaction rate ratio of synthesis to hydrolysis (Vs/Vh), penicillin acylase from Alcaligenes faecalis was chosen as the suitable catalyst for the synthesis of cefaclor. The reaction conditions, such as temperature, pH, and substrate concentration were investigated based on their Vs/Vh values. In the process of preparing cefaclor, in situ product removal (ISPR) and acyl donor feeding were used to achieve high yield. At the optimal conditions, the yield of cefaclor was 90%. In addition, the product were separated and purified, the total yield of cefaclor was 61%.

Citation

Ye-Wang Zhang, Dong-Zhi Wei. Enzymatic preparation of cefaclor with immobilized penicillin acylase. Preparative biochemistry & biotechnology. 2008;38(2):129-38

Mesh Tags

Substances

PMID: 18320464

search → result