Complete genomic characterization of an intertypic Sabin 3/Sabin 2 capsid recombinant.

Evaggelos Dedepsidis, Vaia Pliaka, Zaharoula Kyriakopoulou, Christos Brakoulias, Stamatina Levidiotou-Stefanou, Anastassia Pratti, Zissis Mamuris, Panayotis Markoulatos

Department of Biochemistry and Biotechnology, University of Thessaly, Greece.

FEMS immunology and medical microbiology 2008 Apr

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The genetic properties of strain K/2002, isolated from fecal samples of a 7-month-old child who had received his first oral poliovirus vaccine (OPV) dose at the age of 3 months, are described. Preliminary sequencing characterization of isolate K/2002 revealed an S3/S2 recombination event at the 3' end of the VP1 coding region. A recombination event resulted in the introduction of six Sabin 2 amino acid residues in a Sabin 3 genomic background. Furthermore, mutations associated with loss of the attenuated phenotype of Sabin 3 strains have been identified in the genome of isolate K/2002. The data presented here emphasize the need for careful planning of vaccination strategies, which involve stopping OPV administration in regions that are certified to be polio-free.

Citation

Evaggelos Dedepsidis, Vaia Pliaka, Zaharoula Kyriakopoulou, Christos Brakoulias, Stamatina Levidiotou-Stefanou, Anastassia Pratti, Zissis Mamuris, Panayotis Markoulatos. Complete genomic characterization of an intertypic Sabin 3/Sabin 2 capsid recombinant. FEMS immunology and medical microbiology. 2008 Apr;52(3):343-51