Dcytb (Cybrd1) functions as both a ferric and a cupric reductase in vitro.

Steven Wyman, Robert J Simpson, Andrew T McKie, Paul A Sharp

King's College London, Iron Metabolism Group, Nutritional Sciences Division, Franklin-Wilkins Building, 150 Stamford Street, London SE1 9NH, United Kingdom.

FEBS letters 2008 Jun 11

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MDCK cells expressing an inducible duodenal cytochrome b-green fluorescent protein (Dcytb-EGFP) fusion construct were used to investigate the function of Dcytb. The Dcytb-EGFP protein was targeted correctly to the plasma membrane, and cells displayed increased ferric and cupric reductase activities, which were greatly reduced in the presence of doxycycline. The data suggests that Dcytb plays a physiological role in both iron and copper uptake, through divalent metal transporter 1 (DMT1) and copper transporter 1, respectively. In support of this hypothesis, we show that 59Fe uptake was significantly enhanced in Dcytb-EGFP expressing MDCK cells which endogenously express DMT1.

Citation

Steven Wyman, Robert J Simpson, Andrew T McKie, Paul A Sharp. Dcytb (Cybrd1) functions as both a ferric and a cupric reductase in vitro. FEBS letters. 2008 Jun 11;582(13):1901-6