Enantioselective analysis of oxybutynin and N-desethyloxybutynin with application to an in vitro biotransformation study.

Patrícia da Fonseca, Luis Alexandre Pedro de Freitas, Luis Felipe Ribeiro Pinto, Cezar Rangel Pestana, Pierina Sueli Bonato

Faculdade de Ciências Farmacâeuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2008 Nov 1

filter terms:

An enantioselective method using liquid-phase microextraction (LPME) followed by HPLC analysis was developed for the determination of oxybutynin (OXY) and its major metabolite N-desethyloxybutynin (DEO) in rat liver microsomal fraction. The LPME procedure was optimized using multifactorial experiments. Under the optimal extraction conditions, the mean recoveries were 61 and 55% for (R)-OXY and (S)-OXY, respectively, and 70 and 76% for (R)-DEO and (S)-DEO, respectively. The validated method was employed to an in vitro biotransformation study using rat liver microsomal fraction. The results demonstrated the enantioselective biotransformation of OXY.

Citation

Patrícia da Fonseca, Luis Alexandre Pedro de Freitas, Luis Felipe Ribeiro Pinto, Cezar Rangel Pestana, Pierina Sueli Bonato. Enantioselective analysis of oxybutynin and N-desethyloxybutynin with application to an in vitro biotransformation study. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 2008 Nov 1;875(1):161-7