Synthesis and evaluation of the luciferase-oligodeoxynucleotide for the sequence-selective detection of nucleic acids.

Fumi Nagatsugi, Ryusuke Nakahara, Kaori Inoue, Shigeki Sasaki

Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai, Japan.

Archiv der Pharmazie 2008 Sep

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A new method for the synthesis of ODN-luciferase conjugate was investigated as a signal-amplifying sensor of the target nucleic acids. The conjugation of the luciferase was successfully achieved between the cysteine residue and the 2-amino-6-vinylpurine nucleoside of the ODN probe without significant inactivation of luciferase. The ODN-luciferase conjugate modified with PEG retained the luciferase activity and selectivity during the hybridization with the target ODN.

Citation

Fumi Nagatsugi, Ryusuke Nakahara, Kaori Inoue, Shigeki Sasaki. Synthesis and evaluation of the luciferase-oligodeoxynucleotide for the sequence-selective detection of nucleic acids. Archiv der Pharmazie. 2008 Sep;341(9):562-7