Eco1-dependent cohesin acetylation during establishment of sister chromatid cohesion.

Tom Rolef Ben-Shahar, Sebastian Heeger, Chris Lehane, Philip East, Helen Flynn, Mark Skehel, Frank Uhlmann

Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln'sInn Fields, London WC2A 3PX, UK.

Science (New York, N.Y.) 2008 Jul 25

filter terms:

Replicated chromosomes are held together by the chromosomal cohesin complex from the time of their synthesis in S phase onward. This requires the replication fork-associated acetyl transferase Eco1, but Eco1's mechanism of action is not known. We identified spontaneous suppressors of the thermosensitive eco1-1 allele in budding yeast. An acetylation-mimicking mutation of a conserved lysine in cohesin's Smc3 subunit makes Eco1 dispensable for cell growth, and we show that Smc3 is acetylated in an Eco1-dependent manner during DNA replication to promote sister chromatid cohesion. A second set of eco1-1 suppressors inactivate the budding yeast ortholog of the cohesin destabilizer Wapl. Our results indicate that Eco1 modifies cohesin to stabilize sister chromatid cohesion in parallel with a cohesion establishment reaction that is in principle Eco1-independent.

Citation

Tom Rolef Ben-Shahar, Sebastian Heeger, Chris Lehane, Philip East, Helen Flynn, Mark Skehel, Frank Uhlmann. Eco1-dependent cohesin acetylation during establishment of sister chromatid cohesion. Science (New York, N.Y.). 2008 Jul 25;321(5888):563-6