Modulation of hydrolysis and transglycosylation activity of Thermus maltogenic amylase by combinatorial saturation mutagenesis.

Su-Won Oh, Myoung-Uoon Jang, Chang-Ku Jeong, Hye-Jeong Kang, Jung-Mi Park, Tae-Jip Kim

Department of Food Science and Technology, Chungbuk National University, Cheongju 361-763, Korea.

Journal of microbiology and biotechnology 2008 Aug

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The roles of conserved amino acid residues (Val329-Ala330- Asn331-Glu332), constituting an extra sugar-binding space (ESBS) of Thermus maltogenic amylase (ThMA), were investigated by combinatorial saturation mutagenesis. Various ThMA mutants were firstly screened on the basis of starch hydrolyzing activity and their enzymatic properties were characterized in detail. Most of the ThMA variants showed remarkable decreases in their hydrolyzing activity, but their specificity against various substrates could be altered by mutagenesis. Unexpectedly, mutant H-16 (Gly-Leu-Val-Tyr) showed almost identical hydrolyzing and transglycosylation activities to wild type, whereas K-33 (Ser-Gly-Asp-Glu) showed an extremely low transglycosylation activity. Interestingly, K-33 produced glucose, maltose, and acarviosine from acarbose, whereas ThMA hydrolyzed acarbose to only glucose and acarviosine-glucose, which proposes that the substrate specificity, or hydrolysis or transglycosylation activity of ThMA can be modulated by combinatorial mutations near the ESBS.

Citation

Su-Won Oh, Myoung-Uoon Jang, Chang-Ku Jeong, Hye-Jeong Kang, Jung-Mi Park, Tae-Jip Kim. Modulation of hydrolysis and transglycosylation activity of Thermus maltogenic amylase by combinatorial saturation mutagenesis. Journal of microbiology and biotechnology. 2008 Aug;18(8):1401-7