Diagnosis and treatment of mycoplasma-contaminated cell cultures.

Helena Harlin, Thomas F Gajewski

University of Chicago, Chicago, Illinois, USA.

Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.] 2008 Jan

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Mycoplasma contamination is a serious and frequent problem in the culture laboratory. Although mycoplasma contamination may be suspected by the failure of cells to thrive, the formal diagnosis rests on the detection of adenosine phosphorylase secretion by infected cell lines. This appendix describes how to test for mycoplasma contamination, and also presents methods for antibiotic treatment of infected cultures. Copyright 2008 by John Wiley & Sons, Inc.

Citation

Helena Harlin, Thomas F Gajewski. Diagnosis and treatment of mycoplasma-contaminated cell cultures. Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.]. 2008 Jan;Appendix 3:Appendix 3C