Bisphosphonate mRNA cap analog attached to Sepharose for affinity chromatography of decapping enzymes.

Sylwia A Szczepaniak, Jacek Jemielity, Joanna Zuberek, Joanna Kufel, Edward Darzynkiewicz

Division of Biophysics, Institute of Experimental Physics, University of Warsaw, 02-089 Warsaw, Poland.

Nucleic acids symposium series (2004) 2008

filter terms:

m(7)GTP-Sepharose is routinely used for cap binding protein isolation. Here we present the synthesis of a new affinity resin containing a mononucleotide cap analog resistant to hydrolysis by DcpS. The resin has been designed in order to identify and purify Arabidopsis thaliana DcpS and other pyrophosphatases. The binding efficiency of the new resin to eIF4E protein was compared with standard m(7)GTP-Sepharose. The utility of non-hydrolysable resin was demonstrated on yeast extract.

Citation

Sylwia A Szczepaniak, Jacek Jemielity, Joanna Zuberek, Joanna Kufel, Edward Darzynkiewicz. Bisphosphonate mRNA cap analog attached to Sepharose for affinity chromatography of decapping enzymes. Nucleic acids symposium series (2004). 2008(52):295-6