Correlation Engine 2.0
Clear Search sequence regions

  • Ago1 (1)
  • cell cycle (2)
  • cells (2)
  • Cid14 (4)
  • clr4 (1)
  • dna (2)
  • fission yeast (2)
  • gene (2)
  • nucleotides (2)
  • polymerase rnas (1)
  • regions (1)
  • rnas (21)
  • tramp complex (1)
  • tRNA (1)
  • uracil (1)
  • Sizes of these terms reflect their relevance to your search.

    In the fission yeast Schizosaccharomyces pombe, the RNA interference (RNAi) machinery is required to generate small interfering RNAs (siRNAs) that mediate heterochromatic gene silencing. Efficient silencing also requires the TRAMP complex, which contains the noncanonical Cid14 poly(A) polymerase and targets aberrant RNAs for degradation. Here we use high-throughput sequencing to analyze Argonaute-associated small RNAs (sRNAs) in both the presence and absence of Cid14. Most sRNAs in fission yeast start with a 5' uracil, and we argue these are loaded most efficiently into Argonaute. In wild-type cells most sRNAs match to repeated regions of the genome, whereas in cid14Delta cells the sRNA profile changes to include major new classes of sRNAs originating from ribosomal RNAs and a tRNA. Thus, Cid14 prevents certain abundant RNAs from becoming substrates for the RNAi machinery, thereby freeing the RNAi machinery to act on its proper targets.


    Marc Bühler, Noah Spies, David P Bartel, Danesh Moazed. TRAMP-mediated RNA surveillance prevents spurious entry of RNAs into the Schizosaccharomyces pombe siRNA pathway. Nature structural & molecular biology. 2008 Oct;15(10):1015-23

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 18776903

    View Full Text