Identification of novel synthetic peptide showing angiogenic activity in human endothelial cells.

Chang Hee Lee, Mi-Sook Lee, Sun-Jin Kim, Young-Tae Je, Sung Ho Ryu, Taehoon Lee

SIGMOL Institute of Molecular Medicine, SIGMOL Inc., 790-834 Pohang, South Korea.

Peptides 2009 Feb

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A novel synthetic hexapeptide (SFKLRY-NH(2)) that displays angiogenic activity has been identified by positional scanning of a synthetic peptide combinatorial library (PS-SPCL). The peptide induced proliferation, migration, and capillary-like tube formation in primary cultured HUVECs, and augmented vessel sprouting ex vivo while attenuated by the treatment with pertussis toxin (PTX) or U73122 (PLC-inhibitor) suggesting the influence of PTX-sensitive G-proteins and PLC. In addition, SFKLRY-NH(2) up-regulated the expression of VEGF-A in HUVECs and the neutralizing antibody against VEGF suppressed SFKLRY-NH(2)-induced tube formation activity. Taken together, these results suggest that SFKLRY-NH(2) may induce blood vessel formation by PTX-sensitive G protein-coupled receptor-PLC-Ca(2+) signaling cascade leading into VEGF-A expression in HUVECs.

Citation

Chang Hee Lee, Mi-Sook Lee, Sun-Jin Kim, Young-Tae Je, Sung Ho Ryu, Taehoon Lee. Identification of novel synthetic peptide showing angiogenic activity in human endothelial cells. Peptides. 2009 Feb;30(2):409-18