Arthur V Cresce, James N Culver, William E Bentley, Peter Kofinas
Fischell Department of Bioengineering, University of Maryland, 1120 Jeong H. Kim Building 225, College Park, MD 20742, USA.
Acta biomaterialia 2009 MarThis paper examines the interaction between a block copolymer and a virus. A poly(styrene-b-4-vinylpyridine) block copolymer was loaded with nickel, and cast from a selective solvent mixture to form a cylindrical microstructure (PS/P4VP-Ni). The nickel ions were confined within the P4VP block of the copolymer. The binding of tobacco mosaic virus (TMV) and tobacco necrosis virus on microphase-separated PS/P4VP-Ni was examined. A staining technique was developed to simultaneously visualize virus and block copolymer structure by transmission electron microscopy. Electron microscopy revealed virus particles associated with block copolymer microphase-separated domains, even after extensive washes with Tween. In contrast, virus associated with PS/P4VP block copolymers lacking Ni were readily removed by Tween. The cylinder long axis of the microstructure was oriented using a hot press and a cooled channel die for quenching, resulting in PS/P4VP cylinders that had a strong anisotropic directional preference. When exposed to flowing solutions of TMV, the PS/P4VP-Ni surface exhibited an ability to retain TMV in a partially aligned state, when the direction of flow coincided with the long axis of the PS/P4VP-Ni cylinders. These results suggest that Coulombic interactions provide a robust means for the binding of virus particles to block copolymer surfaces.
Arthur V Cresce, James N Culver, William E Bentley, Peter Kofinas. Block copolymer nanotemplating of tobacco mosaic and tobacco necrosis viruses. Acta biomaterialia. 2009 Mar;5(3):893-902
PMID: 19010745
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