Jesús Ramírez-Santos, Verónica García-Mata, Sebastian Poggio, Laura Camarena, M Carmen Gómez-Eichelmann
Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, P.O. Box 70-228, 04510 Mexico, D.F., Mexico.
Archives of microbiology 2009 FebIn RecBCD(+) cells, a mutated single-strand DNA 3'-5' exonuclease ExoI (SbcB15) induced an increase in stationary-phase mutation. In sbcB15 cells, as in wild-type cells, these mutations partially required RecA, RecB, RecF, and expression of the LexA regulon. The absence of nuclease SbcCD in sbcB15 cells decreased stationary-phase mutation and induced an increase in the number of cell filaments. The absence of ExoI (Deltaxon) in wild-type or sbcC cells did not change significantly the stationary-phase mutation. Differences between the sbcB15 and DeltaxonA cells suggest a correlation between level of SOS induction and the generation of stationary-phase mutations.
Jesús Ramírez-Santos, Verónica García-Mata, Sebastian Poggio, Laura Camarena, M Carmen Gómez-Eichelmann. Role of single-strand DNA 3'-5' exonuclease ExoI and nuclease SbcCD in stationary-phase mutation in Escherichia coli K-12. Archives of microbiology. 2009 Feb;191(2):185-90
PMID: 19018518
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