Generation of human Fab antibody against platelet membrane glycoprotein IIb/IIIa and its effect on platelet aggregation].

Ning-Zheng Dong, Yu-Jie Cui, Chang-Geng Ruan

The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Ministry of Health, Suzhou, China. ningzheng_dong@yahoo.com.cn

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 2009 Jan

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To generation of human Fab fragment against GP GPIIb/IIIa from phage display library and to study its effect on platelet aggregation. An antibody phage display library was constructed from spleen cells from a donor with idiopathic thrombocytopenic purpura(ITP) whose anti-GPIIb/IIIa anti body was positive. High-affinity human mAbs was selected by panning against GPIIb/IIIa expressed on CHO123 cells. The binding specificity of phage antibody to GPIIb/IIIa was detected by ELISA and Western blot. And the effect of antibody on platelet aggregation was analyzed. After three rounds of panning with CHO123, phage antibodies against GPIIb/IIIa were enriched specifically. 2 positive phage antibodies with high specific for GP IIb/IIIa were verified, and the purified antibody can inhibited ADP induced platelet aggregation. Human antibodies against GPIIb/IIIa are obtained from phage display library.

Citation

Ning-Zheng Dong, Yu-Jie Cui, Chang-Geng Ruan. Generation of human Fab antibody against platelet membrane glycoprotein IIb/IIIa and its effect on platelet aggregation]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 2009 Jan;25(1):65-7