Guang Guo, He Xiang Wang, Tzi Bun Ng
State Key Laboratory for Agrobiotechnology and College of Biological Science, China Agricultural University, Beijing, 100094, China.
Protein and peptide letters 2009A new antifungal peptide designated as pomegranin, with an N-terminal sequence resembling that of rice disease resistance NB-S-LRR-like protein, was isolated from fresh pomegranate peels by ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, and gel filtration by fast protein liquid chromatography on Superdex 75. Pomegranin was unadsorbed on DEAE-cellulose but adsorbed on Affi-gel blue gel. It exhibited a molecular mass of 11 kDa in both gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It inhibited mycelial growth in the fungi Botrytis cinerea and Fusarium oxysporum with an IC(50) of 2 microM and 6.1 microM, respectively. It was devoid of hemagglutinating, ribonuclease, deoxyribonuclease and protease inhibitory activities.
Guang Guo, He Xiang Wang, Tzi Bun Ng. Pomegranin, an antifungal peptide from pomegranate peels. Protein and peptide letters. 2009;16(1):82-5
PMID: 19149678
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