Dissection of the critical binding determinants of cellular retinoic acid binding protein II by mutagenesis and fluorescence binding assay.

Chrysoula Vasileiou, Kin Sing Stephen Lee, Rachael M Crist, Soheila Vaezeslami, Sarah M Goins, James H Geiger, Babak Borhan

Proteins 2009 Aug 01

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The binding of retinoic acid to mutants of Cellular Retinoic Acid Binding Protein II (CRABPII) was evaluated to better understand the importance of the direct protein/ligand interactions. The important role of Arg111 for the correct structure and function of the protein was verified and other residues that directly affect retinoic acid binding have been identified. Furthermore, retinoic acid binding to CRABPII mutants that lack all previously identified interacting amino acids was rescued by providing a carboxylic acid dimer partner in the form of a Glu residue. 2008 Wiley-Liss, Inc.

Citation

Chrysoula Vasileiou, Kin Sing Stephen Lee, Rachael M Crist, Soheila Vaezeslami, Sarah M Goins, James H Geiger, Babak Borhan. Dissection of the critical binding determinants of cellular retinoic acid binding protein II by mutagenesis and fluorescence binding assay. Proteins. 2009 Aug 01;76(2):281-90