Flavonoids inhibit the AU-rich element binding of HuC.

Hojoong Kwak, Kyung-Chae Jeong, Min-Ju Chae, Soo-Youl Kim, Woong-Yang Park

Departments of Biomedical Sciences, Biochemistry and Molecular Biology, Seoul National University, College of Medicine, Seoul 110-799, Korea.

BMB reports 2009 Jan 31

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Post-transcriptional regulation of mRNA stability by Hu proteins is an important mechanism for tumorigenesis. We focused on the molecular interactions between the HuC protein and AU-rich elements (AREs) to find chemical inhibitors of RNA-protein interactions using RNA electrophoretic mobility shift assay with non-radioactive probes. Screening of 52 natural compounds identified 14 candidate compounds that displayed potent inhibitory activity. Six (quercetin, myricetin, (-)-epigallocatechin gallate, ellagic acid, (-)-epicatechin gallate, and rhamnetin) were categorized as phytochemicals, and their IC(50) values were low (0.2-1.8 microM). [BMB reports 2009; 42(1): 41-46].

Citation

Hojoong Kwak, Kyung-Chae Jeong, Min-Ju Chae, Soo-Youl Kim, Woong-Yang Park. Flavonoids inhibit the AU-rich element binding of HuC. BMB reports. 2009 Jan 31;42(1):41-6