Yonggang Wang, Fengrong Zhu, Jie Chen, Fusen Han, Heyao Wang
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China.
Biological & pharmaceutical bulletin 2009 MarOxidative stress plays an important role in tissue damage associated with many different chronic degenerative diseases, such as cancer and cardiovascular disorders. In our previous study, a novel dipeptide Pro-Arg derived from protamine hydrolysates was found to possess significant antioxidative activity in vitro. In this study, we compared the antioxidant capabilities of purified and synthesized dipeptide (Pro-Arg) and found both of them had very strong hydroxyl-radical scavenging activity in vitro, even at a very low concentration. The protective effect of the dipeptide against oxidative stress was evaluated using H2O2-induced oxidative stress of human diploid fibroblasts MRC-5 cell model. Our results showed the dipeptide attenuated H2O2-induced cellular oxidative damage, and normalized S phase arrest of MRC-5 cells exposed to H2O2. Our findings demonstrate that Pro-Arg can protect against oxidative stress/damage and H2O2-induced human diploid fibroblasts cell death.
Yonggang Wang, Fengrong Zhu, Jie Chen, Fusen Han, Heyao Wang. Effects of Pro-Arg, a novel dipeptide derived from protamine hydrolysate on H2O2-induced oxidative stress in human diploid fibroblasts. Biological & pharmaceutical bulletin. 2009 Mar;32(3):389-93
PMID: 19252283
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