Overexpression of CHMP6 induces cellular oncosis and apoptosis in HeLa cells.

Dongxu Fu, Linjie Tian, Zhi Peng, Weiwei Deng, Jinsong Yuan, Dalong Ma, Taiping Shi, Dianjun Li, Ying Wang

Laboratory of Medical Immunology, School of Basic Medical Science, Harbin Medical University, Harbin, PR China.

Bioscience, biotechnology, and biochemistry 2009 Mar 23

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Cell death can proceed via at least two distinct pathways, apoptosis and oncosis. Apoptosis is an energy-dependent process characterized morphologically by cell shrinkage, whereas oncosis is defined as a prelethal pathway leading to cell death associated with cellular swelling, organelle swelling, and increased membrane permeability. In this study, we found that overexpression of chromatin modifying protein 6 (CHMP6) induced cell death by a series of experiments, including morphological observation, intracellular ATP determination, caspase-3 activity, and flow cytometry. Typical morphological characteristics consistent with oncosis were observed by transmission electron microscopy. Simultaneously, we obtained some results that indicated apoptosis, but the anti-apoptotic gene Bcl-xL and caspase family inhibitor Z-VAD-FMK had little effect on CHMP6-induced cell death. These results suggest that CHMP6 overexpression can cause cell death, predominantly via oncosis and to a certain extent via apoptosis, and that CHMP6 might be a novel regulator involved in both oncosis and apoptosis.

Citation

Dongxu Fu, Linjie Tian, Zhi Peng, Weiwei Deng, Jinsong Yuan, Dalong Ma, Taiping Shi, Dianjun Li, Ying Wang. Overexpression of CHMP6 induces cellular oncosis and apoptosis in HeLa cells. Bioscience, biotechnology, and biochemistry. 2009 Mar 23;73(3):494-501