Development of a method for the determination of vardenafil in human plasma by high performance liquid chromatography with UV detection.

Giuseppe Carlucci, Paola Palumbo, Piera Iuliani, Giancarlo Palumbo

Dipartimento di Scienze del Farmaco, Facoltà di Farmacia, Università degli Studi G. D'Annunzio, Chieti-Pescara, Chieti, Italy. g.carlucci@unich.it

Biomedical chromatography : BMC 2009 Jul

filter terms:

A simple high-performance liquid chromatographic (HPLC) method with photometric detection is described for the determination of vardenafil hydrochloride, a phosphodiesterase V inhibitor, in human plasma. Chromatographic separation of the analyte and internal standard was achieved on an analytical 250 x 4.6 mm i.d. reversed-phase Kromasil KR 100 C(18) (5 microm particle size) column using a mobile phase of acetonitrile-potassium dihydrogen phosphate (30:70 v/v). The run time was less than 15 min. Column eluate was monitored at 230 nm. The linearity over the concentration range of 10-1500 ng/mL for vardenafil was obtained and the limit of quantification (LOQ) was 10 ng/mL. The method has been applied to analysis of the vardenafil concentrations for application in pharmacokinetic studies. (c) 2009 John Wiley & Sons, Ltd.

Citation

Giuseppe Carlucci, Paola Palumbo, Piera Iuliani, Giancarlo Palumbo. Development of a method for the determination of vardenafil in human plasma by high performance liquid chromatography with UV detection. Biomedical chromatography : BMC. 2009 Jul;23(7):759-63