Cultivation of fastidious bacteria by viability staining and micromanipulation in a soil substrate membrane system.

B C Ferrari, M R Gillings

Applied and environmental microbiology 2009 May

filter terms:

Soil substrate membrane systems allow for microcultivation of fastidious soil bacteria as mixed microbial communities. We isolated established microcolonies from these membranes by using fluorescence viability staining and micromanipulation. This approach facilitated the recovery of diverse, novel isolates, including the recalcitrant bacterium Leifsonia xyli, a plant pathogen that has never been isolated outside the host.

Citation

B C Ferrari, M R Gillings. Cultivation of fastidious bacteria by viability staining and micromanipulation in a soil substrate membrane system. Applied and environmental microbiology. 2009 May;75(10):3352-4