Expression, purification, and characterization of the recombinant putative periplasmic hemin-binding protein (HutB) of Photobacterium damselae subsp. piscicida.

Francesca Andreoni, Romina Boiani, Giordano Serafini, Irene Bianconi, Sabrina Dominici, Francesca Gorini, Mauro Magnani

Department of Biomolecular Science, Section of Biotechnology, University of Urbino Carlo Bo, via T. Campanella, Fano, PU, Italy. francesca.andreoni@uniurb.it

Bioscience, biotechnology, and biochemistry 2009 May

filter terms:

HutB, the periplasmic hemin binding protein of Photobacterium damselae subsp. piscicida, was produced as a recombinant protein. UV-Vis spectrophotometrical analysis showed absorption spectral changes in hemin upon mixing it with the recombinant protein, indicating complex formation. Spectrophotometric titration of HutB with hemin showed saturation at a heme/HutB ratio of 1:1 and a binding affinity (K(d)) of 10 microM.

Citation

Francesca Andreoni, Romina Boiani, Giordano Serafini, Irene Bianconi, Sabrina Dominici, Francesca Gorini, Mauro Magnani. Expression, purification, and characterization of the recombinant putative periplasmic hemin-binding protein (HutB) of Photobacterium damselae subsp. piscicida. Bioscience, biotechnology, and biochemistry. 2009 May;73(5):1180-3