AFLP analysis of genetic diversity of Prunella].

Yufeng Shen, Yiming Sun, Xiaoxia Shen, Jianming Jiang, Zhian Wang, Xuping Yu

College of Agriculture and Biotechnology of Zhengjiang University, Hangzhou 310029, China.

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 2009 Feb

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To explore the variety of the genetic polymorphism of eight Prunella germplasm resources by AFLP analysis. The amplified fragment length polymorphism (AFLP) tags were applied to screen out 32 selective amplification primer pairs, the amplified bands as original matrix were analyzed with NTSYS-PC software for the similarity between the Prunella germplasm and the construction of genetic phylogenetic tree. SDS extraction of genomic Prunella DNA showed a good quality, could meet the requirements of AFLP analysis. From 32 selective amplification primer pairs, 10 pairs with strong polymorphism, better band and higher resolution were used for the construction of the AFLP Prunella fingerprint, all eight Prunella germplasms were separated, they were divided into 3 categories. Prunella germplasm resources are rich in genetic diversity, certain morphological characteristics and differences are associate with genotype.

Citation

Yufeng Shen, Yiming Sun, Xiaoxia Shen, Jianming Jiang, Zhian Wang, Xuping Yu. AFLP analysis of genetic diversity of Prunella]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. 2009 Feb;34(3):260-3