Use of the DNA Checkerboard hybridization method for detection and quantitation of Candida species in oral microbiota.

Ribeirão Preto Dental School, University of São Paulo, Department of Dental Materials and Prosthodontics, Avenida do Café S/N, Monte Alegre, Ribeirão Preto, São Paulo, Brazil.

Canadian journal of microbiology 2009 May

filter terms:

The DNA Checkerboard method enables the simultaneous identification of distinct microorganisms in a large number of samples and employs up to 45 whole genomic DNA probes to gram-negative and gram-positive bacterial species present in subgingival biofilms. Collectively, they account for 55%-60% of the bacteria in subgingival biofilms. In this study, we present the DNA Checkerboard hybridization as an alternative method for the detection and quantitation of Candida species in oral cavities. Our results reveal that DNA Checkerboard is sensitive enough and constitutes a powerful and appropriate method for detecting and quantifying Candida species found in the oral cavity.

Citation

Cássio do Nascimento, Rubens Ferreira de Albuquerque Junior, João Paulo Mardegan Issa, Izabel Yoko Ito, Cláudia Helena Lovato da Silva, Helena de Freitas Oliveira Paranhos, Raphael Freitas de Souza. Use of the DNA Checkerboard hybridization method for detection and quantitation of Candida species in oral microbiota. Canadian journal of microbiology. 2009 May;55(5):622-6