Site-directed mutagenesis identifies a molecular switch involved in copper sensing by the histidine kinase CinS in Pseudomonas putida KT2440.

Davide Quaranta, Megan M McEvoy, Christopher Rensing

Department of Soil, Water, and Environmental Science, University of Arizona, Tucson, AZ 85721, USA.

Journal of bacteriology 2009 Aug

filter terms:

In the presence of copper, Pseudomonas putida activates transcription of cinAQ via the two-component system CinS-CinR. The CinS-CinR TCS was responsive to 0.5 microM copper and was specifically activated only by copper and silver. Modeling studies of CinS identified a potential copper binding site containing H37 and H147. CinS mutants with H37R and H147R mutations had an almost 10-fold reduced copper-dependent induction of cinAQ compared to the wild type.

Citation

Davide Quaranta, Megan M McEvoy, Christopher Rensing. Site-directed mutagenesis identifies a molecular switch involved in copper sensing by the histidine kinase CinS in Pseudomonas putida KT2440. Journal of bacteriology. 2009 Aug;191(16):5304-11