A phenotypic study of TFS1 mutants differentially altered in the inhibition of Ira2p or CPY.

Aurélie Gombault, Jonas Warringer, Robert Caesar, Fabienne Godin, Béatrice Vallée, Michel Doudeau, Hélène Chautard, Anders Blomberg, Hélène Bénédetti

Centre de Biophysique Moléculaire, Centre National de la Recherche Scientifique, UPR 4301, Affiliated to the University of Orléans and INSERM, Orléans, France.

FEMS yeast research 2009 Sep

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The Saccharomyces cerevisiae protein Tfs1p is known as a dual protein. On the one hand, it inhibits the carboxypeptidase Y protease, and on the other, it inhibits Ira2p, a GTPase-activating protein of Ras. We managed to dissect precise areas of Tfs1p specifically involved in only one of those functions. Based on these data, specific Tfs1p point mutants affected in only one of these two functions were constructed. In order to obtain insights on the physiological role of these functions, systematic phenotypic tests were performed on strains expressing these specific Tfs1p mutants. The results obtained demonstrate that the inhibition of Ira2p by Tfs1p is the predominant function under the conditions tested.

Citation

Aurélie Gombault, Jonas Warringer, Robert Caesar, Fabienne Godin, Béatrice Vallée, Michel Doudeau, Hélène Chautard, Anders Blomberg, Hélène Bénédetti. A phenotypic study of TFS1 mutants differentially altered in the inhibition of Ira2p or CPY. FEMS yeast research. 2009 Sep;9(6):867-74