Attenuation of LPS-induced iNOS expression by 1,5-anhydro-d-fructose.

Xiaojie Meng, Ko-ichi Kawahara, Kenji Matsushita, Yuko Nawa, Binita Shrestha, Kiyoshi Kikuchi, Hisayo Sameshima, Teruto Hashiguchi, Ikuro Maruyama

Department of Laboratory and Vascular Medicine Cardiovascular and Respiratory Disorders Advanced Therapeutics, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima, Japan.

Biochemical and biophysical research communications 2009 Sep 11

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1,5-anhydro-d-fructose (1,5-AF), a monosaccharide formed from starch and glycogen, exhibits antioxidant and antibacterial activity, and inhibits cytokine release by attenuating NF-kappaB activation in LPS-stimulated mice. The present study examined whether 1,5-AF inhibits lipopolysaccharide (LPS)-induced inducible nitric oxide synthase (iNOS) in vitro and in vivo. We found that 1,5-AF significantly blocked the production of NO, and protein and mRNA expression of iNOS, and up-regulated IL-10 production in vitro. We also investigated the effects of 1,5-AF on acute lung inflammation in C57BL/6J mice. We found that protein and mRNA expression of iNOS in lung tissues were inhibited by 1,5-AF pretreatment. In addition, the serum level of IL-10 was upregulated by 1,5-AF. Collectively, the iNOS transcriptional and translational inhibitory effects of 1,5-AF seem to be prolonged and enhanced by the production of IL-10. These results suggest that 1,5-AF could be a useful adjunct in the treatment of acute lung inflammation.

Citation

Xiaojie Meng, Ko-ichi Kawahara, Kenji Matsushita, Yuko Nawa, Binita Shrestha, Kiyoshi Kikuchi, Hisayo Sameshima, Teruto Hashiguchi, Ikuro Maruyama. Attenuation of LPS-induced iNOS expression by 1,5-anhydro-d-fructose. Biochemical and biophysical research communications. 2009 Sep 11;387(1):42-6