Characterization of short-lived intermediates produced during replication of baculovirus DNA.

Victor S Mikhailov, George F Rohrmann

N. K. Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia. mikhailov48@mail.ru

Virus research 2009 Oct

filter terms:

In this report the short-lived DNA replication intermediates produced in both uninfected and Autographa californica multiple nucleopolyhedrovirus (AcMNPV) infected Spodoptera frugiperda cells were characterized. The methods used included pulse-labeling of DNA in permiabilized cells, treatment of nascent DNA with Mung bean nuclease, and electrophoresis in neutral and alkaline agarose gels. In contrast to uninfected cells that produced a population of small DNA fragments of about 200bp, a population of heterogeneous fragments of up to 5kb with an average size of 1-2kb derived randomly from the virus genome was identified as the short-lived intermediates produced during AcMNPV replication. The intermediates likely include Okazaki fragments derived from the lagging strands in viral replication forks as well as fragments produced during the recombination-dependent replication.

Citation

Victor S Mikhailov, George F Rohrmann. Characterization of short-lived intermediates produced during replication of baculovirus DNA. Virus research. 2009 Oct;145(1):106-11