Clear Search sequence regions


  • CBF2 (1)
  • CBF3 (1)
  • cell cycle (2)
  • chromatin (1)
  • Cse4p (3)
  • dna fungal (2)
  • mitosis (2)
  • MTW1 (1)
  • NDC80 (1)
  • nuclear proteins (2)
  • NUF2 (1)
  • Sizes of these terms reflect their relevance to your search.

    The kinetochore is a complex protein-DNA assembly that provides the mechanical linkage between microtubules and the centromere DNA of each chromosome. Centromere DNA in all eukaryotes is wrapped around a unique nucleosome that contains the histone H3 variant CENP-A (Cse4p in Saccharomyces cerevisiae). Here, we report that the inner kinetochore complex (CBF3) is required for pericentric DNA looping at the Cse4p-containing nucleosome. DNA within the pericentric loop occupies a spatially confined area that is radially displaced from the interpolar central spindle. Microtubule-binding kinetochore complexes are not involved in pericentric DNA looping but are required for the geometric organization of DNA loops around the spindle microtubules in metaphase. Thus, the mitotic segregation apparatus is a composite structure composed of kinetochore and interpolar microtubules, the kinetochore, and organized pericentric DNA loops. The linkage of microtubule-binding to centromere DNA-looping complexes positions the pericentric chromatin loops and stabilizes the dynamic properties of individual kinetochore complexes in mitosis.

    Citation

    Marybeth Anderson, Julian Haase, Elaine Yeh, Kerry Bloom. Function and assembly of DNA looping, clustering, and microtubule attachment complexes within a eukaryotic kinetochore. Molecular biology of the cell. 2009 Oct;20(19):4131-9

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 19656849

    View Free Full Text