Chemoporation using saponins or cholates: an alternative method for transformation of bacterial cells.

Matjaz Ravnikar, Andreja Irman, Natasa Radić, Mojca Lunder, Borut Strukelj

Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Askerceva 7, 1000, Ljubljana, Slovenia. matjaz.ravnikar@ffa.uni-lj.si

Biotechnology letters 2009 Dec

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A new method for fast transformation of competent bacterial cells has been developed. The transformation is induced with cholic acid analogues or saponins which cause reversible disruption of the bacterial membrane. This method shortens the time of transformation without significant loss of transformation efficiency in comparison to heat shock method and is the first reported chemically-induced transformation. New data about interactions between cholates and biomembranes is revealed that may contribute to better understanding of bacterial transformation.

Citation

Matjaz Ravnikar, Andreja Irman, Natasa Radić, Mojca Lunder, Borut Strukelj. Chemoporation using saponins or cholates: an alternative method for transformation of bacterial cells. Biotechnology letters. 2009 Dec;31(12):1943-6