Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice.

Konstantinos Anastassiadis, Jun Fu, Christoph Patsch, Shengbiao Hu, Stefanie Weidlich, Kristin Duerschke, Frank Buchholz, Frank Edenhofer, A Francis Stewart

Center for Regenerative Therapies Dresden, BioInnovationsZentrum Technische Universitaet Dresden, Am Tatzberg 47, 01307 Dresden, Germany.

Disease models & mechanisms 2009 Sep-Oct

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Tyrosine site-specific recombinases (SSRs) including Cre and FLP are essential tools for DNA and genome engineering. Cre has long been recognized as the best SSR for genome engineering, particularly in mice. Obtaining another SSR that is as good as Cre will be a valuable addition to the genomic toolbox. To this end, we have developed and validated reagents for the Dre-rox system. These include an Escherichia coli-inducible expression vector based on the temperature-sensitive pSC101 plasmid, a mammalian expression vector based on the CAGGs promoter, a rox-lacZ reporter embryonic stem (ES) cell line based on targeting at the Rosa26 locus, the accompanying Rosa26-rox reporter mouse line, and a CAGGs-Dre deleter mouse line. We also show that a Dre-progesterone receptor shows good ligand-responsive induction properties. Furthermore, we show that there is no crossover recombination between Cre-rox or Dre-loxP. Hence, we add another set of efficient tools to the genomic toolbox, which will enable the development of more sophisticated mouse models for the analysis of gene function and disease.

Citation

Konstantinos Anastassiadis, Jun Fu, Christoph Patsch, Shengbiao Hu, Stefanie Weidlich, Kristin Duerschke, Frank Buchholz, Frank Edenhofer, A Francis Stewart. Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice. Disease models & mechanisms. 2009 Sep-Oct;2(9-10):508-15