Regulation of mu opioid receptor internalization by the scaffold protein RanBPM.

Jeffery N Talbot, Donald A Skifter, Elisabetta Bianchi, Daniel T Monaghan, Myron L Toews, L Charles Murrin

Department of Pharmacology and Experimental Neuroscience, 985800 Nebraska Medical Center, Omaha, NE 68198-5800, USA.

Neuroscience letters 2009 Dec 11

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Mu opioid receptors (MOP) are transducers of the pharmacological effects of many opioid drugs, including analgesia and tolerance/dependence. Previously, we observed increased MOP signaling during postnatal development that was not associated with increased MOP or G protein expression. A yeast two-hybrid screen of a human brain cDNA library using the MOP C-terminus as bait identified RanBPM as a potential MOP-interacting protein. RanBPM has been recognized as a multi-functional scaffold protein that interacts with a variety of signaling receptors/proteins. Co-immunoprecipitation studies in HEK293 cells indicated that RanBPM constitutively associates with MOP. Functionally, RanBPM had no effect on MOP-mediated inhibition of adenylyl cyclase, yet reduced agonist-induced endocytosis of MOP. Mechanistically, RanBPM interfered with beta arrestin2-GFP translocation stimulated by MOP but not alpha(1B)-adrenergic receptor activation, indicating selectivity of action. Our findings suggest that RanBPM is a novel MOP-interacting protein that negatively regulates receptor internalization without altering MOP signaling through adenylyl cyclase.

Citation

Jeffery N Talbot, Donald A Skifter, Elisabetta Bianchi, Daniel T Monaghan, Myron L Toews, L Charles Murrin. Regulation of mu opioid receptor internalization by the scaffold protein RanBPM. Neuroscience letters. 2009 Dec 11;466(3):154-8