Differential immunoproteomics enables identification of Metarhizium anisopliae proteins related to Rhipicephalus microplus infection.

Lucélia Santi, Walter Orlando Beys da Silva, Antônio Frederico Michel Pinto, Augusto Schrank, Marilene Henning Vainstein

Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, P.O. Box 15005, CEP 91501-970, Porto Alegre, RS, Brazil.

Research in microbiology 2009 Dec

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Differential immunoproteomics was applied to identify proteins secreted by Metarhizium anisopliae induced by the Rhipicephalus microplus cuticle. In addition, IgG anti-spore surface proteins were used for searching for proteins possibly involved in early stages of fungus versus tick infection. LC-MS/MS of differentially secreted proteins led to the identification of proteases (carboxypeptidase and Pr1A), chitinase, carboxylic acid transport and proline-rich protein. Differential immunoproteomics strategy facilitated the detection and the identification of new proteins related to M. anisopliae host-pathogen interaction and could be used in further works to identify novel proteins related to other microbial infection systems.

Citation

Lucélia Santi, Walter Orlando Beys da Silva, Antônio Frederico Michel Pinto, Augusto Schrank, Marilene Henning Vainstein. Differential immunoproteomics enables identification of Metarhizium anisopliae proteins related to Rhipicephalus microplus infection. Research in microbiology. 2009 Dec;160(10):824-8