FLAG-tag selective covalent protein labeling via a binding-induced acyl-transfer reaction.

Hiroshi Nonaka, Sho-hei Fujishima, Sho-hei Uchinomiya, Akio Ojida, Itaru Hamachi

Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Kyoto 615-8510, Japan.

Bioorganic & medicinal chemistry letters 2009 Dec 1

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A FLAG tag selective protein labeling method is newly developed in this study. Coupling of the selective binding between synthetic Ni-complex probe and FLAG tag with the acyl transfer reaction enables the site-selective covalent modification of FLAG peptide and FLAG-tag fused protein.

Citation

Hiroshi Nonaka, Sho-hei Fujishima, Sho-hei Uchinomiya, Akio Ojida, Itaru Hamachi. FLAG-tag selective covalent protein labeling via a binding-induced acyl-transfer reaction. Bioorganic & medicinal chemistry letters. 2009 Dec 1;19(23):6696-9