Noriko Konishi, Hiromi Obata, Yukako Shimojima, Chie Monma, Akemi Kai, Takao Tsuji
Department of Microbiology, Tokyo Metropolitan Institute of Public Health.
KansenshÅgaku zasshi. The Journal of the Japanese Association for Infectious Diseases 2009 SepA box-lunch-associated food-borne outbreak occurred in Tokyo and Chiba Prefecture in June 2003 involved six types of enterotoxigenic Escherichia coli (ETEC). Fecal specimens from patients were screened for ETEC using colony-sweep polymerase chain reaction (PCR). Of the 84 fecal specimens examined, 56 (66.7%) were PCR-positive, i.e. 35 (41.7%) LT-gene-positive, 21 (25.0%) STp-gene-positive and 11 (13.1%) STh-gene-positive. Both of toxin-genes, i.e. LT and STp, LT and STh, STh and STp were positive in 11 patients. ETEC was isolated in confirmation testing from 48 (57.1%) fecal specimens. A single type of ETEC was isolated from 43 fecal samples. Serotype and toxin type of the isolates were O25:NM (LT) (21 samples), O27:H20 (STp) (12 samples), O148:H28 (STh) (8 samples), O25:NM (STh) (1 sample), and O27:7 (STp) (1 sample). Two types of ETEC were isolated from 5 fecal samples, i.e. O25:NM (LT) and O27:H20 (STp) (3 samples), O27:H20 (STp) and O148:H28 (STh) (1 sample), and O25:NM (LT) and O78:NM (STh) (1 sample).
Noriko Konishi, Hiromi Obata, Yukako Shimojima, Chie Monma, Akemi Kai, Takao Tsuji. Colony-sweep PCR efficacy in food-borne outbreak associated with six types of enterotoxigenic Escherichia coli]. KansenshÅgaku zasshi. The Journal of the Japanese Association for Infectious Diseases. 2009 Sep;83(5):490-5
PMID: 19860248
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