Muscleblind-like 1 is a negative regulator of TGF-beta-dependent epithelial-mesenchymal transition of atrioventricular canal endocardial cells.

Natalie A Vajda, Kyle R Brimacombe, Kathryn E LeMasters, Andrea N Ladd

Department of Cell Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA.

Developmental dynamics : an official publication of the American Association of Anatomists 2009 Dec

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The development of the valves and septa of the heart depends on the formation and remodeling of endocardial cushions. Here, we report that the alternative splicing regulator muscleblind-like 1 (MBNL1) exhibits a regionally restricted pattern of expression in canal region endocardium and ventricular myocardium during endocardial cushion development in chicken. Knockdown of MBNL1 in atrioventricular explants leads to a transforming growth factor beta-dependent increase in epithelial-mesenchymal transition (EMT) of endocardial cells. This reveals a novel role for MBNL1 during embryonic development, and represents the first evidence that an alternative splicing regulator is a key player in endocardial cushion development. (c) 2009 Wiley-Liss, Inc.

Citation

Natalie A Vajda, Kyle R Brimacombe, Kathryn E LeMasters, Andrea N Ladd. Muscleblind-like 1 is a negative regulator of TGF-beta-dependent epithelial-mesenchymal transition of atrioventricular canal endocardial cells. Developmental dynamics : an official publication of the American Association of Anatomists. 2009 Dec;238(12):3266-72