Surface-energy dependent contact activation of blood factor XII.

Avantika Golas, Purnendu Parhi, Ziad O Dimachkie, Christopher A Siedlecki, Erwin A Vogler

Department of Bioengineering, The Pennsylvania State University, University Park, PA 16802, USA.

Biomaterials 2010 Feb

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Contact activation of blood factor XII (FXII, Hageman factor) in neat-buffer solution exhibits a parabolic profile when scaled as a function of silanized-glass-particle activator surface energy (measured as advancing water adhesion tension tau(a)(o)=gamma(lv)(o)cos theta in dyne/cm, where gamma(lv)(o) is water interfacial tension in dyne/cm and theta is the advancing contact angle). Nearly equal activation is observed at the extremes of activator water-wetting properties -36cm (55 degrees FXII-procoagulant contact. Results suggest that materials falling within the 20blood-plasma coagulation through the intrinsic pathway. Surface chemistries falling within this range are, however, a perplexingly difficult target for surface engineering because of the critical balance that must be struck between hydrophobicity and hydrophilicity. Results are interpreted within the context of blood plasma coagulation and the role of water and proteins at procoagulant surfaces. (c) 2009 Elsevier Ltd. All rights reserved.

Citation

Avantika Golas, Purnendu Parhi, Ziad O Dimachkie, Christopher A Siedlecki, Erwin A Vogler. Surface-energy dependent contact activation of blood factor XII. Biomaterials. 2010 Feb;31(6):1068-79