Decolorization of anthraquinone Vat Blue 4 by the free cells of an autochthonous bacterium, Bacillus subtilis.

Rajee Olaganathan, Jamila Patterson

Suganthi Devadason Marine Research Institute, 44- Beach Road, Tuticorin 628001, Tamil Nadu, India. rajeeom@yahoo.com

Water science and technology : a journal of the International Association on Water Pollution Research 2009

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Uncontaminated soil, Vat Blue 4 contaminated soil and Vat Blue 4 effluent were screened for heterotrophic bacterial population and the bacterial density were found to be 19.3 x 10(4) Colony Forming Units (CFU)/gm, 5.5 x 10(4) CFU/gm and 1.1 x 10(4) CFU/ml respectively. Student's 't' test analysis affirmed that significant variation prevailed between the three set of 't' tests conducted (P<0.001 to 0.002). The heterotrophic bacterial population of dye contaminated soil comprised of 32.5% of Pseudomonas spp. followed by 27.5% of Bacillus spp., 15.0% of Aeromonas spp., 12.5% of Micrococcus spp. and 12.5% of Achromobacter spp. The optimum inoculums load, pH and temperature were found to be 5% (10 x 10(4) counts), 10 and 35 degrees C respectively. Free cells of B. subtilis decolorized Vat Blue 4 up to 92.30% after 24 hours of treatment. Total Dissolved Solids (TDS), Biological Oxygen Demand (BOD(5)) and Chemical Oxygen Demand (COD) were reduced up to 50.00, 79.60 and 75.40% respectively.

Citation

Rajee Olaganathan, Jamila Patterson. Decolorization of anthraquinone Vat Blue 4 by the free cells of an autochthonous bacterium, Bacillus subtilis. Water science and technology : a journal of the International Association on Water Pollution Research. 2009;60(12):3225-32