A simple and rapid method of bacterial transformation.

Gottfried Wilharm, Daniela Lepka, Franziska Faber, Julia Hofmann, Tobias Kerrinnes, Evelyn Skiebe

Robert Koch-Institute, Wernigerode Branch, Burgstr. 37, D-38855 Wernigerode, Germany. wilharmg@rki.de

Journal of microbiological methods 2010 Feb

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Recently, a unique method for bacterial transformation using nanofibers to inoculate DNA has been developed by Naoto Yoshida and colleagues. We have verified the principle, transforming Escherichia coli, Yersinia enterocolitica and Acinetobacter baumannii, and have established a user-friendly protocol. A buffered suspension of sepiolite-an inexpensive, fibrous yet inoffensive mineral-is mixed with bacteria and transforming DNA and the mixture directly spread on selective agar. Copyright 2009 Elsevier B.V. All rights reserved.

Citation

Gottfried Wilharm, Daniela Lepka, Franziska Faber, Julia Hofmann, Tobias Kerrinnes, Evelyn Skiebe. A simple and rapid method of bacterial transformation. Journal of microbiological methods. 2010 Feb;80(2):215-6