Microarray analysis of p-anisaldehyde-induced transcriptome of Saccharomyces cerevisiae.

Lu Yu, Na Guo, Yi Yang, Xiuping Wu, Rizeng Meng, Junwen Fan, Fa Ge, Xuelin Wang, Jingbo Liu, Xuming Deng

Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, 130062, Changchun, China.

Journal of industrial microbiology & biotechnology 2010 Mar

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p-Anisaldehyde (4-methoxybenzaldehyde), an extract from Pimpinella anisum L. seeds, is a potential novel preservative. To reveal the possible action mechanism of p-anisaldehyde against microorganisms, yeast-based commercial oligonucleotide microarrays were used to analyze the genome-wide transcriptional changes in response to p-anisaldehyde. Quantitative real-time RT-PCR was performed for selected genes to verify the microarray results. We interpreted our microarray data with the clustering tool, T-profiler. Analysis of microarray data revealed that p-anisaldehyde induced the expression of genes related to sulphur assimilation, aromatic aldehydes metabolism, and secondary metabolism, which demonstrated that the addition of p-anisaldehyde may influence the normal metabolism of aromatic aldehydes. This genome-wide transcriptomics approach revealed first insights into the response of Saccharomyces cerevisiae (S. cerevisiae) to p-anisaldehyde challenge.

Citation

Lu Yu, Na Guo, Yi Yang, Xiuping Wu, Rizeng Meng, Junwen Fan, Fa Ge, Xuelin Wang, Jingbo Liu, Xuming Deng. Microarray analysis of p-anisaldehyde-induced transcriptome of Saccharomyces cerevisiae. Journal of industrial microbiology & biotechnology. 2010 Mar;37(3):313-22