HBV X protein interacts with cytoskeletal signaling proteins through SH3 binding.

Huixing Feng, Tuan Lin Tan, Dandan Niu, Wei Ning Chen

Frontiers in bioscience (Elite edition) 2010 Jan 01

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The aim of this study was to investigate interactions between cellular SH3-containing proteins and the proline-rich domain in Hepatitis B Virus (HBV) X protein (HBx) The proline-rich domain of HBx (amino acids 19-58) as well as the relevant site-directed mutagenesis (proline to alanine residues) were cloned into pGEX-5X-1 and expressed as GST-PXXP and GST-AXXA probes. Panomics SH3 domain arrays were probed using both GST-PXXP and GST-AXXA to identify potential interacting SH3 domain containing proteins. The specific interactions were confirmed by the immunoprecipitation of the full-length SH3 domain-containing protein. We report here the binding assay which demonstrated interaction between PXXP domain in HBx and the SH3-domain containing proteins, in particular various signaling proteins involved in cytoskeletal reorganization. Our findings were consistent with similar virus-host interactions via SH3 binding for other viruses such as hepatitis C virus (HCV) and human immunodeficiency virus (HIV) Further characterization of the proline-rich binding to SH3 domains could yield important information for the design of novel therapeutic measures against downstream disease causative effects of HBx in the liver cells.

Citation

Huixing Feng, Tuan Lin Tan, Dandan Niu, Wei Ning Chen. HBV X protein interacts with cytoskeletal signaling proteins through SH3 binding. Frontiers in bioscience (Elite edition). 2010 Jan 01;2(1):143-50